• Vaccine Name: S. aureus CNA Protein Vaccine
• Target Pathogen: Staphylococcus aureus
• Target Disease: Staph infections
• Vaccine Ontology ID: VO_0004048
• Type: Subunit vaccine
• Status: Research
• Antigen: a recombinant fragment of CNA protein (Nilsson et al., 1998).
• cna gene engineering:
  • Type: Recombinant protein preparation
  • Description: CNA fragments were expressed in E. coli and then purified (Nilsson et al., 1998).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0000139
• Immunization Route: subcutaneous injection

Mouse Response

• Host Strain: NMRI
• Vaccination Protocol: Purified recombinant collagen adhesin fragments M17, M31, M55, and BSA (Sigma Chemical Co.) were dissolved in PBS and emulsified 1:1 in Freund’s complete adjuvant 200 μl of the emulsion containing 100 μg of protein was injected subcutaneously (s.c.) on day -31. Booster immunizations (s.c.; 100 μg protein in PBS) were performed 15 and 24 d after the initial vaccination (Nilsson et al., 1998).
• Challenge Protocol: After the final boost, mice were inoculated intravenously (i.v.) with S. aureus (Nilsson et al., 1998).
• Efficacy: At 14 d after inoculation, mortality in the collagen adhesin-vaccinated group was only 13%, compared with 87% in the control antigen immunized group (Nilsson et al., 1998).