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Vaccine Detail

S. aureus FnbA Protein Vaccine
Vaccine Information
  • Vaccine Name: S. aureus FnbA Protein Vaccine
  • Target Pathogen: Staphylococcus aureus
  • Target Disease: Staph infections
  • Vaccine Ontology ID: VO_0004049
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: Fibronectin binding domain of FnbA protein
  • fnbA gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: complete Freunds adjuvant
  • Immunization Route: subcutaneous injection
Host Response

Mouse Response

  • Host Strain: BSVS
  • Vaccination Protocol: Mice were vaccinated subcutaneously in the neck region with 20 μg of the antigens emulsified with complete Freund's adjuvant (primary vaccination) a few days after mating. Booster doses were given after 14 days with the same amount of immunogen, but emulsified with incomplete Freund's adjuvant (Mamo et al., 1994).
  • Challenge Protocol: Mice were challenged 18-21 days after the booster dose by intramammary inoculation (mouse mastitis model) 23. Briefly, mice were anaesthetized using ether inhalation, the teat tips were aseptically removed and a 0.1 ml bacterial suspension (1 x l0^6 c.f.u, m1-1) was inoculated into the left quarter (L-4) and right quarter (R-4) mouse mammary glands (Mamo et al., 1994).
  • Efficacy: Vaccination of mice with recombinant FnBP resulted in significant protection against challenge with S. aureus (Mamo et al., 1994).
References
Mamo et al., 1994: Mamo W, Jonsson P, Flock JI, Lindberg M, Müller HP, Wadström T, Nelson L. Vaccination against Staphylococcus aureus mastitis: immunological response of mice vaccinated with fibronectin-binding protein (FnBP-A) to challenge with S. aureus. Vaccine. 1994; 12(11); 988-992. [PubMed: 7975852].