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Vaccine Detail

M. tuberculosis DNA Vaccine encoding KatG
Vaccine Information
  • Vaccine Name: M. tuberculosis DNA Vaccine encoding KatG
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0004058
  • Type: DNA vaccine
  • Status: Research
  • KatG gene engineering:
    • Type: DNA vaccine construction
    • Description: KatG protein was amplified from M. tuberculosis H37Rv chromosomal DNA by PCR, and then cloned into the eukaryotic expression vector pJW4303 (Li et al., 1999).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pJW4303 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: C57BL/6
  • Vaccination Protocol: The C57BL/6 mice (five mice per group) were vaccinated intramuscularly into the thigh three times at 3-week intervals, with 100 μg of plasmid DNA per injection. Control mice were vaccinated with 106 CFU of BCG Pasteur injected subcutaneously (Li et al., 1999).
  • Challenge Protocol: The mice were challenged by the aerosol route about 17 to 21 days after receiving the final DNA vaccine inoculation and 6 weeks after receiving the BCG vaccination with M. tuberculosis Erdman (Li et al., 1999).
  • Efficacy: Vaccination with the KatG resulted in a 75 to 85% drop in number of lung bacteria. Moreover, mice immunized with the KatG TPA-positive constructs showed significantly decreased dissemination of the lung infection to the spleen (Li et al., 1999).
  • Information about this animal model: Mouse Model for TB research
References
Li et al., 1999: Li Z, Howard A, Kelley C, Delogu G, Collins F, Morris S. Immunogenicity of DNA vaccines expressing tuberculosis proteins fused to tissue plasminogen activator signal sequences. Infection and immunity. 1999; 67(9); 4780-4786. [PubMed: 10456931].