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Vaccine Detail

M. tuberculosis HBHA Protein Vaccine
Vaccine Information
  • Vaccine Name: M. tuberculosis HBHA Protein Vaccine
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0004059
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: HBHA
  • HBHA gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: MPL vaccine adjuvant
  • Adjuvant: DDA vaccine adjuvant
  • Immunization Route: subcutaneous injection
Host Response

Mouse Response

  • Host Strain: C57BL/6
  • Vaccination Protocol: were vaccinated subcutaneously three times with 5 μg of either HPLC-purified HBHA (nHBHA) or recombinant HBHA emulsified in 150 μg of dimethyldioctadecylammonium bromide (DDA) (Acros Organics, Morris Plains, N.J.)-monophosphoryl lipid A (MPL) adjuvant or with the adjuvant combination alone (Parra et al., 2004).
  • Challenge Protocol: Mice were challenged with aerosolized M. tuberculosis Erdman (200 CFU) 4 weeks following the final immunization (Parra et al., 2004).
  • Efficacy: The HBHA-containing vaccine gave a approximately 0.7-log reduction in CFU in both mouse lungs and spleens compared to adjuvant controls 28 days following challenge (Parra et al., 2004).
  • Host Ifng (Interferon gamma) response
    • Description: There was a ~10-fold increase in the amount of IFN-γ released in the ex vivo assay by spleen cells from HBHA-immunized mice compared to cells from naïve mice or mice immunized with only adjuvant, which was a significantly higher amount. Splenocytes from immunized mice were incubated with murine BMMΦ infected with M. bovis BCG for 72 hours. Lymph node-derived lymphocytes from HBHA-vaccinated mice released approximately eightfold more IFN-γ in the presence of HBHA-stimulated macrophages than cells from naïve or adjuvant control mice released, which was also significant (Parra et al., 2004).
    • Detailed Gene Information: Click Here.
  • Information about this animal model: Mouse Model for TB research
References
Parra et al., 2004: Parra M, Pickett T, Delogu G, Dheenadhayalan V, Debrie AS, Locht C, Brennan MJ. The mycobacterial heparin-binding hemagglutinin is a protective antigen in the mouse aerosol challenge model of tuberculosis. Infection and immunity. 2004; 72(12); 6799-6805. [PubMed: 15557600].