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Vaccine Detail

H. pylori HspA Protein Vaccine
Vaccine Information
  • Vaccine Name: H. pylori HspA Protein Vaccine
  • Target Pathogen: Helicobacter pylori
  • Target Disease: Ulcers
  • Vaccine Ontology ID: VO_0004060
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: Recombinant HspA protein
  • hspA gene engineering:
    • Type: Recombinant protein preparation
    • Description: The hspA gene was cloned into the expression vector pMAL-C2 and expressed in E. coli as a MalE fusion (Ferrero et al., 1995).
    • Detailed Gene Information: Click Here.
  • Adjuvant: cholera toxin
    • Adjuvant name: cholera toxin
    • VO adjuvant ID: VO_0000143
    • Description: 5 μg of cholera toxin (Ferrero et al., 1995).
  • Immunization Route: Orally
Host Response

Mouse Response

  • Host Strain: Swiss
  • Vaccination Protocol: Mice were orogastrically immunized with 50 μg of recombinant HspA protein and 5 μg of cholera toxin prepared in 0.1 M sodium bicarbonate before delivery (Ferrero et al., 1995).
  • Challenge Protocol: Mice were challenged with 104 H. felis bacteria (Ferrero et al., 1995).
  • Efficacy: Orogastric immunization of mice with recombinant H. pylori HspA proteins protected 80% of animals from a challenge dose of 104 Helicobacter felis bacteria (Ferrero et al., 1995).

Mouse Response

  • Host Strain: Swiss
  • Vaccination Protocol: Mice were orogastrically immunized with 50 μg of recombinant HspB protein and 5 μg of cholera toxin prepared in 0.1 M sodium bicarbonate before delivery (Ferrero et al., 1995).
  • Challenge Protocol: Mice were challenged with 104 H. felis bacteria (Ferrero et al., 1995).
  • Efficacy: Orogastric immunization of mice with recombinant H. pylori HspB proteins protected 70% of animals from a challenge dose of 104 Helicobacter felis bacteria (Ferrero et al., 1995).
References
Ferrero et al., 1995: Ferrero RL, Thiberge JM, Kansau I, Wuscher N, Huerre M, Labigne A. The GroES homolog of Helicobacter pylori confers protective immunity against mucosal infection in mice. Proceedings of the National Academy of Sciences of the United States of America. 1995; 92(14); 6499-6503. [PubMed: 7604021].