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Vaccine Detail

H. pylori HspB Protein Vaccine
Vaccine Information
  • Vaccine Name: H. pylori HspB Protein Vaccine
  • Target Pathogen: Helicobacter pylori
  • Target Disease: Ulcers
  • Vaccine Ontology ID: VO_0004061
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: Recombinant HspB protein
  • hspB gene engineering:
    • Type: Recombinant protein preparation
    • Description: The hspB gene was cloned into the expression vector pMAL-C2 and expressed in E. coli as a MalE fusion
    • Detailed Gene Information: Click Here.
  • Adjuvant: cholera toxin
    • Adjuvant name: cholera toxin
    • VO adjuvant ID: VO_0000143
    • Description: 5 μg of cholera toxin (Ferrero et al., 1995).
  • Immunization Route: Orally
Host Response

Mouse Response

  • Host Strain: Swiss pathogen free
  • Vaccination Protocol: Antigen extracts (50 μg of protein) containing 5 μg of cholera toxin were used to immunize mice orogastrically (Ferrero et al., 1995).
  • Challenge Protocol: Aliquots (0.1 ml) containing 104 H. felis bacteria prepared from a low subculture stock suspension of H. felis were administered orogastrically to mice (Ferrero et al., 1995).
  • Efficacy: Orogastric immunization of mice with recombinant H. pylori HspB proteins protected 70% of animals from a challenge dose of 104 Helicobacter felis bacteria (Ferrero et al., 1995).
References
Ferrero et al., 1995: Ferrero RL, Thiberge JM, Kansau I, Wuscher N, Huerre M, Labigne A. The GroES homolog of Helicobacter pylori confers protective immunity against mucosal infection in mice. Proceedings of the National Academy of Sciences of the United States of America. 1995; 92(14); 6499-6503. [PubMed: 7604021].