• Vaccine Name: P. chabaudi AMA1 Protein Vaccine
• Target Pathogen: Plasmodium spp.
• Target Disease: Malaria
• Vaccine Ontology ID: VO_0004194
• Type: Subunit vaccine
• Status: Research
• Antigen: Recombinant ectodomain of P. chabaudi adami (DS stain) AMA1 (denoted rAMA1B)
• AMA-1 from P. chabaudi gene engineering:
  • Type: Recombinant protein preparation
  • Description:
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001268
  • Description: Montanide ISA720
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Groups of three to five mice were immunized i.p. with 15 µg of rAMA1B emulsified in Montanide ISA720. Four weeks later, a booster immunization was given using the same amount of rAMA1B emulsified with Montanide ISA720. Controls were immunized with PBS emulsified in Montanide ISA720 (Xu et al., 2000).
• Challenge Protocol: Ten days after being given a booster immunization the mice were challenged i.v. with 1 x 10^5 P. chabaudi adami parasitized erythrocytes (Xu et al., 2000).
• Efficacy: Immunized mice demonstrated significantly lower peak parasitemias compared with PBS-immunized mice, showing that rAMA1B immunization confers protection against challenge with P. chabaudi (Xu et al., 2000).