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Vaccine Detail

P. falciparum DNA Vaccine encoding EBA-175
Vaccine Information
  • Vaccine Name: P. falciparum DNA Vaccine encoding EBA-175
  • Target Pathogen: Plasmodium spp.
  • Target Disease: Malaria
  • Vaccine Ontology ID: VO_0004196
  • Type: DNA vaccine
  • Status: Research
  • Antigen: P. falciparum EBA-175 RII
  • eba-175 gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pVR1020 DNA vaccine plasmid
  • Immunization Route: Intradermal injection (i.d.)
Host Response

Monkey Response

  • Host Strain: Aotus lemurinus lemurinus
  • Vaccination Protocol: Intradermal delivery of DNA vaccines was performed under light sedation with Ketamine at 20 mg/kg intramuscularly, using a 1 mL insulin syringe with a fused 29-gauge 0.5-inch needle. Monkeys received a total of 500 μg of plasmid DNA in saline in a series of four immunizations at weeks 0, 3, 6, and 47 on the lower back on six different sites. The maximal volume administered in any one site was 100 μl (Sim et al., 2001).
  • Challenge Protocol: Aotus monkeys were challenged with 1 X 104 P. falciparum (FVO) infected erythrocytes (Sim et al., 2001).
  • Efficacy: One of three monkeys vaccinated with EBA-175 was protected from challenge of parasitized erythrocytes (Sim et al., 2001).
References
Sim et al., 2001: Sim BK, Narum DL, Liang H, Fuhrmann SR, Obaldia N 3rd, Gramzinski R, Aguiar J, Haynes JD, Moch JK, Hoffman SL. Induction of biologically active antibodies in mice, rabbits, and monkeys by Plasmodium falciparum EBA-175 region II DNA vaccine. Molecular medicine (Cambridge, Mass.). 2001; 7(4); 247-254. [PubMed: 11471569].