• Vaccine Name: VVr expressing L. infantum P36/LACK
• Target Pathogen: Leishmania infantum
• Target Disease: Infantile visceral leishmaniasis
• Vaccine Ontology ID: VO_0004198
• Type: Recombinant vector vaccine
• Status: Research
• Antigen: L. infantum p36/LACK protein
• P36/LACK gene engineering:
  • Type: Recombinant vector construction
  • Description: The gene encoding L. infantum p36/LACK protein was expressed by recombinant vaccinia virus, and co-expressed murine IL-12 (Gonzalo et al., 2001).
  • Detailed Gene Information: Click Here.
• Adjuvant:
  • Adjuvant name:
  • VO adjuvant ID: VO_0001147
• Immunization Route: Intraperitoneal injection (i.p.)

Mouse Response

• Host Strain: BALB/c
• Vaccination Protocol: Mice were primed with VVr (5 × 10^7 PFU/mouse) by the intraperitoneal (i.p.) route. Two weeks later (14 days p.i.), animals were boosted with VVr or recombinant p36 antigen (Gonzalo et al., 2001).
• Challenge Protocol: 35 days p.i., mice were challenged in the right hind foot with 10^5 of L. major stationary-phase promastigote culture (Gonzalo et al., 2001).
• Efficacy: BALB/c mice immunized with a DNA vector expressing p36/LACK of Leishmania infantum followed by a booster with VVp36/LACK induced significant protection against Leishmania major infection (Gonzalo et al., 2001).