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Vaccine Detail

L. infantum SMT Protein Vaccine
Vaccine Information
  • Vaccine Name: L. infantum SMT Protein Vaccine
  • Target Pathogen: Leishmania infantum
  • Target Disease: Infantile visceral leishmaniasis
  • Vaccine Ontology ID: VO_0004066
  • Type: Subunit vaccine
  • Status: Research
  • SMT gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: MPL vaccine adjuvant
  • Immunization Route: subcutaneous injection
Host Response

Mouse Response

  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were immunized with 10 μg of rSMT plus 20 μg of MPL®-SE in a volume of 0.1 ml. Another group of mice was administrated with 10 μg of rSMT alone. Control groups received either saline or MPL®-SE alone. The mice were immunized subcutaneously three times at three weeks intervals in the right hind footpad and at the base of the tail (Goto et al., 2007).
  • Challenge Protocol: Mice were challenged with L. infantum three weeks after the last immunization. 5 × 106 L. infantum promastigotes were suspended in Hank's balanced salt solution and injected i.v. into the tail vein of the mouse (Goto et al., 2007).
  • Efficacy: Significant reduction of parasites was seen in mice immunized with rSMT plus MPL®-SE compared with those in saline or adjuvant alone groups. The immunized mice showed 43-fold and 55-fold reduction in the number of parasites in spleens, 111-fold and 117-fold reduction in livers compared with saline and adjuvant alone groups, respectively (Goto et al., 2007).
References
Goto et al., 2007: Goto Y, Bogatzki LY, Bertholet S, Coler RN, Reed SG. Protective immunization against visceral leishmaniasis using Leishmania sterol 24-c-methyltransferase formulated in adjuvant. Vaccine. 2007; 25(42); 7450-7458. [PubMed: 17804125].