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Vaccine Detail

C. muridarum Tarp subunit vaccine
Vaccine Information
  • Vaccine Name: C. muridarum Tarp subunit vaccine
  • Target Pathogen: Chlamydia muridarum
  • Vaccine Ontology ID: VO_0004202
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: purified C. muridarum Tarp
  • Tarp gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: CpG DNA vaccine adjuvant
  • Adjuvant: incomplete Freunds adjuvant
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Mice were immunized intramuscularly with purified C. muridarum Tarp emulsified in CPG-IFA adjuvant or just adjuvant (as a control). Mice were immunized in the hind legs 3 times with an interval of 3 weeks between the first and second injections and 9 days between the second and third injections (Wang et al., 2009).
  • Challenge Protocol: Thirty days after the third immunization, each mouse was inoculated intravaginally with 1 × 104 IFUs of live C. muridarum organisms in 20μl of SPG (sucrose-phosphate-glutamate buffer). Five days prior to infection, each mouse was injected with 2.5mg Depo-provera subcutaneously to synchronize menstrual cycle and increase mouse susceptibility to chlamydial infection (Wang et al., 2009).
  • Efficacy: Immunization of mice with Tarp induced Th1-dominant immunity that significantly reduced the shedding of live organisms from the lower genital tract and attenuated inflammatory pathologies in the fallopian tube tissues. Tarp, an immunodominant antigen identified by human antisera, can induce protective immunity against chlamydial infection and pathology in mice (Wang et al., 2009).
References
Wang et al., 2009: Wang J, Chen L, Chen F, Zhang X, Zhang Y, Baseman J, Perdue S, Yeh IT, Shain R, Holland M, Bailey R, Mabey D, Yu P, Zhong G. A chlamydial type III-secreted effector protein (Tarp) is predominantly recognized by antibodies from humans infected with Chlamydia trachomatis and induces protective immunity against upper genital tract pathologies in mice. Vaccine. 2009; 27(22); 2967-2980. [PubMed: 19428908].