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Vaccine Detail

rBCG-Ag85A[Tokyo]
Vaccine Information
  • Vaccine Name: rBCG-Ag85A[Tokyo]
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0000565
  • Type: Live, attenuated vaccine
  • Antigen: BCG and Mycobacterium antigen Ag85A (Sugawara et al., 2007).
  • FbpA (Ag85A) gene engineering:
    • Type: Recombinant vector construction
    • Description: The Ag85A gene was amplified by PCR and subcloned into the pCR4 vector. The gene was inserted into the pBBN vector (Ag85A-HA) possessing a hemagglutinin (HA) tag at its 5′ end. The Ag85A-HA was expressed in Escherichia coli. The Ag85A-HA gene was then introduced into the downstream region of the pHPS integration vector. The vector was then electroporated into BCG Tokyo. The resulting recombinant was named (rBCG-Ag85A[Tokyo]) (Sugawara et al., 2007).
    • Detailed Gene Information: Click Here.
Host Response

Guinea pig Response

  • Host Strain: Hartley female guinea pigs
  • Vaccination Protocol: Hartley female guinea pigs (group 2) were vaccinated with BCG Tokyo (Sugawara et al., 2007).
References
Sugawara et al., 2007: Sugawara I, Li Z, Sun L, Udagawa T, Taniyama T. Recombinant BCG Tokyo (Ag85A) protects cynomolgus monkeys (Macaca fascicularis) infected with H37Rv Mycobacterium tuberculosis. Tuberculosis (Edinburgh, Scotland). 2007 Aug 24; ; . [PubMed: 17720625 ].