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Vaccine Detail

M-44
Vaccine Information
  • Vaccine Name: M-44
  • Target Pathogen: Coxiella burnetii
  • Target Disease: Q fever
  • Vaccine Ontology ID: VO_0004131
  • Type: Live, attenuated vaccine
  • Preparation: Crude rickettsial pools are produced in embryonated eggs of hens free from avian leucosis virus. The embryonated eggs are inoculated with 0.2 ml of seed stock containing 107.9 median infectious doses at 35C. After 6 to 7 days, the yolk sacs are collected and diluted to a 50% suspension with distilled water. The strains are then obtained through purification using alternating low-speed and high-speed centrifugations. After centrifugation, the pellet is suspended in 10% sucrose and centrifuged onto a 70% sucrose solution. The purified strains are then obtained from the surface and diluted with Snyder I buffer to result in a final titer of 109.5 median infectious dose per ml and freeze-dried (Robinson and Hasty, 1974).
  • Description: M-44 vaccine is developed from attenuated phase II M-44 strain of C. burnetii (Robinson and Hasty, 1974).
Host Response

Guinea pig Response

  • Host Strain: Hartley guinea pigs
  • Vaccination Protocol: Hartley guinea pigs (250 to 350 g) were immunized intraperitoneally or subcutaneously with serial 10-fold dilutions (prepared in physiological buffered saline) of M-44 vaccine (Robinson and Hasty, 1974).
  • Immune Response: Vaccine at high doses prevented anamnestic-type response (Robinson and Hasty, 1974).
  • Challenge Protocol: Guinea pigs were challenged with either phase I or phase II C. burnetii (Robinson and Hasty, 1974).
  • Efficacy: 102 median infectious dose protected the guinea pigs from death, but did not protect the animals from febrile effects of challenge. Protections against the febrile effects of the challenge of phase I C. burnetii were observed at vaccines doses of 104 median infectious dose or greater. Protections against the febrile effects of the challenge of phase II C. burnetii were observed at vaccine doses of 103 median infectious dose or greater (Robinson and Hasty, 1974).
References
Robinson and Hasty, 1974: Robinson DM, Hasty SE. Production of a potent vaccine from the attenuated M-44 strain of Coxiella burneti. Applied microbiology. 1974; 27(4); 777-783. [PubMed: 4825980 ].