VIOLIN Logo
VO Banner
Search: for Help
About
Introduction
Statistics
VIOLIN News
Your VIOLIN
Register or Login
Submission
Tutorial
Vaccine & Components
Vaxquery
Vaxgen
VBLAST
Protegen
VirmugenDB
DNAVaxDB
CanVaxKB
Vaxjo
Vaxvec
Vevax
Huvax
Vaccine Mechanisms
Vaximmutordb
Vaxism
Vaxar
Vaccine Literature
VO-SciMiner
Litesearch
Vaxmesh
Vaxlert
Vaccine Design
Vaxign
Community Efforts
Vaccine Ontology
ICoVax 2012
ICoVax 2013
Advisory Committee
Vaccine Society
Vaxperts
VaxPub
VaxCom
VaxLaw
VaxMedia
VaxMeet
VaxFund
VaxCareer
Data Exchange
V-Utilities
VIOLINML
Help & Documents
Publications
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

Vaccine Detail

T. gondii DNA vaccine encoding MIC8
Vaccine Information
  • Vaccine Name: T. gondii DNA vaccine encoding MIC8
  • Target Pathogen: Toxoplasma gondii
  • Target Disease: Toxoplasmosis
  • Vaccine Ontology ID: VO_0004219
  • Type: DNA vaccine
  • Status: Research
  • Antigen: MIC8
  • MIC8 gene engineering:
    • Type: DNA vaccine construction
    • Description: To construct the pVAX-MIC8 expression plasmid, the coding sequence of the T. gondii MIC8 gene was amplified by polymerase chain reaction (PCR) from genomic DNA of T. gondii. The PCR product was cloned in pGEM-T easy vector (Promega, USA) and sequenced in both directions to ensure fidelity and generated pGEM-MIC8. The MIC8 fragment was inserted into the BamH I/Xba I sites of pVAX I by double digestion with BamH I/Xba I restriction enzymes, and ligation was finally done using T4 DNA ligase. The resulting plasmid was named pVAX-MIC8 (Liu et al., 2010).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pVAX1 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Host Strain: Kunming
  • Vaccination Protocol: Mice (20 per group) received injections of 100 μl of pVAXMIC8 plasmid or empty pVAX I vector into each anterior tibial muscle (final plasmid concentration, 100 μg/100 μl). The injections were repeated using the same protocol 2 and 4 weeks after vaccination. Tail bleeds were performed on vaccinated mice on the day of first vaccination, second vaccination, and third vaccination and pre-challenge to ascertain the presence of specific anti-T. gondii antibodies (Liu et al., 2010).
  • Challenge Protocol: Mice in all groups were intraperitoneally challenged with the virulent T. gondii RH strain (103 tachyzoites per mouse) 2 weeks after the last immunization (Liu et al., 2010).
  • Efficacy: Immunization of mice with pVAXMIC8 dramatically increased the survival time (10.3± 0.9 days) compared with control mice which died within 5 days of challenge (Liu et al., 2010).
References
Liu et al., 2010: Liu MM, Yuan ZG, Peng GH, Zhou DH, He XH, Yan C, Yin CC, He Y, Lin RQ, Song HQ, Zhu XQ. Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis. Parasitology research. 2010; 106(5); 1079-1084. [PubMed: 20177910].