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Vaccine Detail
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S. aureus IsdB subunit vaccine (V710) |
| Vaccine Information |
- Vaccine Name: S. aureus IsdB subunit vaccine (V710)
- Target Pathogen: Staphylococcus aureus
- Target Disease: Staph infections
- Product Name: V710
- Vaccine Ontology ID: VO_0004220
- Type: Subunit vaccine
- Status: Research
- Antigen: Purified IsdB protein
- Adjuvant:
- Immunization Route: Intramuscular injection (i.m.)
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| Host Response |
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Mouse Response
- Host Strain: BALB/c
- Vaccination Protocol: Groups of 20 BALB/c or ICR mice were immunized three times with IsdB (20 μg per dose) formulated with amorphous aluminum hydroxyphosphate sulfate adjuvant (AAHSA) (450 μg per dose) or were sham immunized with AAHSA alone. The doses were administered as two 50-μl intramuscular injections on days 0, 7, and 21(Kuklin et al., 2006).
- Challenge Protocol: 35 days after vaccination, mice were challenged with an 80 to 90% lethal dose (LD80-90) of S. aureus Becker (4.9 × 10^8 to 8.7 × 10^8 CFU for BALB/c mice and 1.0 × 10^9 to 2.0 × 10^9 CFU for ICR mice), and survival was monitored for 10 days. (Kuklin et al., 2006).
- Efficacy: Mice immunized with IsdB exhibited greater survival (45, 40, 32, 29, 40, and 40%) than the mice in the sham-immunized control group (Kuklin et al., 2006).
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| References |
Kuklin et al., 2006: Kuklin NA, Clark DJ, Secore S, Cook J, Cope LD, McNeely T, Noble L, Brown MJ, Zorman JK, Wang XM, Pancari G, Fan H, Isett K, Burgess B, Bryan J, Brownlow M, George H, Meinz M, Liddell ME, Kelly R, Schultz L, Montgomery D, Onishi J, Losada M, Martin M, Ebert T, Tan CY, Schofield TL, Nagy E, Meineke A, Joyce JG, Kurtz MB, Caulfield MJ, Jansen KU, McClements W, Anderson AS. A novel Staphylococcus aureus vaccine: iron surface determinant B induces rapid antibody responses in rhesus macaques and specific increased survival in a murine S. aureus sepsis model. Infection and immunity. 2006; 74(4); 2215-2223. [PubMed: 16552052].
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