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Vaccine Detail

YopE(67-77) Protein Vaccine
Vaccine Information
  • Vaccine Name: YopE(67-77) Protein Vaccine
  • Target Pathogen: Yersinia pestis
  • Target Disease: Plague
  • Vaccine Ontology ID: VO_0004273
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: YopE(69-77) peptide (Lin et al., 2011).
  • YopE gene engineering:
    • Type: Recombinant protein preparation
    • Description: YopE(69-77) protein was sythezised by New England Peptide (Gardner, MA) (Lin et al., 2011).
    • Detailed Gene Information: Click Here.
  • Adjuvant: cholera toxin
  • Immunization Route: intranasal immunization
Host Response

Mouse Response

  • Host Strain: C57BL/6
  • Vaccination Protocol: Mice were lightly anesthetized by isoflurane and immunized intranasally with a 15 μl saline solution containing 1 or 10 μg peptide and 1 μg cholera toxin (CT; List Biological Laboratory, Campbell, CA). Mice were immunized on days 0, 7, and 21, and challenged with Y. pestis strain D27 on day 37 or day 56 (Lin et al., 2011).
  • Challenge Protocol: Mice were lightly anesthetized by isoflurane and infected intranasally with 20 or 200 median lethal doses (MLD) Y. pestis strain D27 in 30 μl saline. The intranasal MLD of Y. pestis strain D27 is ∼1 x 104 CFU when the bacteria are grown and administered, as described above (Lin et al., 2011).
  • Efficacy: 83% of mice immunized with YopE(69-77) peptide survived lethal challenge with Y. pestis D27 (Lin et al., 2011).
References
Lin et al., 2011: Lin JS, Szaba FM, Kummer LW, Chromy BA, Smiley ST. Yersinia pestis YopE Contains a Dominant CD8 T Cell Epitope that Confers Protection in a Mouse Model of Pneumonic Plague. Journal of immunology (Baltimore, Md. : 1950). 2011; ; . [PubMed: 21653834].