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Vaccine Detail

Actinobacillus pleuropneumoniae apxIIIB and apxIIID double mutant vaccine
Vaccine Information
  • Vaccine Name: Actinobacillus pleuropneumoniae apxIIIB and apxIIID double mutant vaccine
  • Target Pathogen: Actinobacillus pleuropneumoniae
  • Target Disease: porcine pleuropneumonia
  • Type: Live, attenuated vaccine
  • Status: Research
  • apxIIIB gene engineering:
    • Type: Gene mutation
    • Description:
    • Detailed Gene Information: Click Here.
  • apxIIID gene engineering:
    • Type: Gene mutation
    • Description:
    • Detailed Gene Information: Click Here.
  • Preparation: An apxIIIBD mutant (1536∆B∆D) was selected and the correct mutation was verified by PCR using three primer pairs. The X3BD-F2 and X3BD-R2 primer pair was designed to produce products of 3720 and 4365 bps from the wild-type 1536 and 1536∆B∆D genomes, respectively (Park et al., 2009).
  • Immunization Route: intranasal immunization
Host Response

Pig Response

  • Persistence: The LD50 of 1536∆B∆D in mice was 6.9 x 108 CFU, while that of the wild-type was 4.0 x 108 CFU, showing that the mutation of the apxIIIBD genes attenuates the lethality of A. pleuropneumoniae in mice (Park et al., 2009).
  • Efficacy: To evaluate the ability of the mutant 1536∆B∆D strain to protect mice against lethal challenge, mice were immunized with 1536∆B∆D twice and challenged with a lethal dose of wild-type A. pleuropneumoniae 1536. Eight of the nine mice immunized with 1536∆B∆D survived for more than 72 hr after lethal challenge (89% protection), while all eight of the unimmunized mice died by 72 hr after challenge (Park et al., 2009).
References
Park et al., 2009: Park C, Ha Y, Kim S, Chae C, Ryu DY. Construction and characterization of an Actinobacillus pleuropneumoniae serotype 2 mutant lacking the Apx toxin secretion protein genes apxIIIB and apxIIID. The Journal of veterinary medical science / the Japanese Society of Veterinary Science. 2009; 71(10); 1317-1323. [PubMed: 19887737].