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Vaccine Detail

Actinobacillus pleuropneumoniae apxIIC and apxIVA double deletion mutant vaccine
Vaccine Information
  • Vaccine Name: Actinobacillus pleuropneumoniae apxIIC and apxIVA double deletion mutant vaccine
  • Target Pathogen: Actinobacillus pleuropneumoniae
  • Target Disease: porcine pleuropneumonia
  • Type: Live, attenuated vaccine
  • Status: Research
  • ApxIIC gene engineering:
    • Type: Gene mutation
    • Description:
    • Detailed Gene Information: Click Here.
  • ApxIVA gene engineering:
    • Type: Gene mutation
    • Description:
    • Detailed Gene Information: Click Here.
  • Preparation: Plasmid pENT1 was used to introduce the ΔapxIVA deletion into A. pleuropneumoniae single mutant ΔapxIIC via the single-step transconjugation system. The colonies with the correct PCR profile were confirmed by Southern blot and sequencing assays (Liu et al., 2007).
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Persistence: The LD50 data shown that the double mutant ΔapxIICΔapxIVA was attenuated by three-fold, compared with the single mutant HB04C− (Liu et al., 2007).
  • Efficacy: Two weeks after secondary immunization, all mice were challenged with 10 LD50 of homologous (WF83) (6.0 × 107) and heterologous (1.8 × 106) (S4074) virulent A. pleuropneumonaie by intraperitoneal route. There was no mouse died in groups 1 and 2, which shown a protection efficiency of 100% against homologous challenge (Liu et al., 2007).
References
Liu et al., 2007: Liu J, Chen X, Lin L, Tan C, Chen Y, Guo Y, Jin M, Guo A, Bei W, Chen H. Potential use an Actinobacillus pleuropneumoniae double mutant strain DeltaapxIICDeltaapxIVA as live vaccine that allows serological differentiation between vaccinated and infected animals. Vaccine. 2007; 25(44); 7696-7705. [PubMed: 17767980].