• Vaccine Name: Mycobacterium tuberculosis sigE mutant vaccine
• Target Pathogen: Mycobacterium tuberculosis
• Target Disease: Tuberculosis
• Vaccine Ontology ID: VO_0002795
• Type: Live, attenuated vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• sigE gene engineering:
  • Type: Gene mutation
  • Description: This sigE mutant is from Mycobacterium tuberculosis (Hernandez et al., 2010).
  • Detailed Gene Information: Click Here.
• Immunization Route: Intratracheal immunization

Mouse Response

• Persistence: A sigE mutant is attenuated in mice (Hernandez et al., 2010).
• Efficacy: A sigE mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hernandez et al., 2010).
• Host Defb3 response
  • Description: A sigE mutant showed a significantly higher and constant expression of the gene encoding β-defensin 3 in the lungs beginning the 7th day post vaccination until the 28th day as compared to mice infected with the parental strain (Hernandez et al., 2010).
  • Detailed Gene Information: Click Here.
• Host Ifng (Interferon gamma) response
  • Description: A sigE mutant showed a significantly higher and constant expression of the gene encoding IFN-γ as compared to mice infected with the parental strain. IFN-gamma was significantly upregulated in mice lungs after vaccination with the mutant as compared to infection with the wild type beginning day 1 post-infection and continuing throughout the duration of the experiment (Hernandez et al., 2010).
  • Detailed Gene Information: Click Here.
• Host Il10 (interleukin 10) response
  • Description: The expression of IL-10 was higher in sigE mutant-infected mice lungs as compared to infection with the parental strain, but it was significant only 21 and 28 days after the infection (Hernandez et al., 2010).
  • Detailed Gene Information: Click Here.
• Host Il4 (interleukin 4) response
  • Description: IL-4 was expressed at a significantly lower level in the sigE mutant-infected mice as compared to mice infected with the wild type pathogen. Significant down regulation of IL-4 by the mutant in mouse lungs was seen days 1-28 post infection as compared to infection with the wild type (Hernandez et al., 2010).
  • Detailed Gene Information: Click Here.
• Host TNF-alpha response
  • Description: A sigE mutant showed a significantly higher and constant expression of the gene encoding TNF-alpha as compared to mice infected with the parental strain. Vaccination with the mutant significantly up-regulated TNF-alpha in mice lungs on days 1, 21, and 28 post-infection as compared to infection with the wild type (Hernandez et al., 2010).
  • Detailed Gene Information: Click Here.
• Information about this animal model: Mouse Model for TB research

Human Response

• Description: The human monocytic cell line THP-1 was obtained from the ATCC, and cultures were grown (Fontán et al., 2008).
• Host APOBEC3B response
  • Description: A sigE mutant upregulates APOBEC3B in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host C19orf66 response
  • Description: A sigE mutant upregulates C19orf66 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host CD70 response
  • Description: A sigE mutant upregulates CD70 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host GPR182 response
  • Description: A sigE mutant upregulates GPR182 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host HIST1H2BE response
  • Description: A sigE mutant upregulates HIST1H2BE in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host HIST2H4A response
  • Description: A sigE mutant upregulates HIST2H4A in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host PCDHB1 response
  • Description: A sigE mutant upregulates PCDHB1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host PTCD1 response
  • Description: A sigE mutant upregulates PTCD1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host STAG3 response
  • Description: A sigE mutant upregulates STAG3 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host TBX21 response
  • Description: A sigE mutant upregulates TBX21 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.
• Host TSC22D4 response
  • Description: A sigE mutant upregulates TSC22D4 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
  • Detailed Gene Information: Click Here.