VIOLIN Logo
VO Banner
Search: for Help
About
Introduction
Statistics
VIOLIN News
Your VIOLIN
Register or Login
Submission
Tutorial
Vaccine & Components
Vaxquery
Vaxgen
VBLAST
Protegen
VirmugenDB
DNAVaxDB
CanVaxKB
Vaxjo
Vaxvec
Vevax
Huvax
Vaccine Mechanisms
Vaximmutordb
Vaxism
Vaxar
Vaccine Literature
VO-SciMiner
Litesearch
Vaxmesh
Vaxlert
Vaccine Design
Vaxign
Community Efforts
Vaccine Ontology
ICoVax 2012
ICoVax 2013
Advisory Committee
Vaccine Society
Vaxperts
VaxPub
VaxCom
VaxLaw
VaxMedia
VaxMeet
VaxFund
VaxCareer
Data Exchange
V-Utilities
VIOLINML
Help & Documents
Publications
Documents
FAQs
Links
Acknowledgements
Disclaimer
Contact Us
UMMS Logo

Vaccine Detail

Mycobacterium tuberculosis sigE mutant vaccine
Vaccine Information
  • Vaccine Name: Mycobacterium tuberculosis sigE mutant vaccine
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0002795
  • Type: Live, attenuated vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • sigE gene engineering:
    • Type: Gene mutation
    • Description: This sigE mutant is from Mycobacterium tuberculosis (Hernandez et al., 2010).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Intratracheal immunization
Host Response

Mouse Response

  • Persistence: A sigE mutant is attenuated in mice (Hernandez et al., 2010).
  • Efficacy: A sigE mutant induces significant protection in mice from challenge with wild type M. tuberculosis (Hernandez et al., 2010).
  • Host Defb3 response
    • Description: A sigE mutant showed a significantly higher and constant expression of the gene encoding β-defensin 3 in the lungs beginning the 7th day post vaccination until the 28th day as compared to mice infected with the parental strain (Hernandez et al., 2010).
    • Detailed Gene Information: Click Here.
  • Host Ifng (Interferon gamma) response
    • Description: A sigE mutant showed a significantly higher and constant expression of the gene encoding IFN-γ as compared to mice infected with the parental strain. IFN-gamma was significantly upregulated in mice lungs after vaccination with the mutant as compared to infection with the wild type beginning day 1 post-infection and continuing throughout the duration of the experiment (Hernandez et al., 2010).
    • Detailed Gene Information: Click Here.
  • Host Il10 (interleukin 10) response
    • Description: The expression of IL-10 was higher in sigE mutant-infected mice lungs as compared to infection with the parental strain, but it was significant only 21 and 28 days after the infection (Hernandez et al., 2010).
    • Detailed Gene Information: Click Here.
  • Host Il4 (interleukin 4) response
    • Description: IL-4 was expressed at a significantly lower level in the sigE mutant-infected mice as compared to mice infected with the wild type pathogen. Significant down regulation of IL-4 by the mutant in mouse lungs was seen days 1-28 post infection as compared to infection with the wild type (Hernandez et al., 2010).
    • Detailed Gene Information: Click Here.
  • Host TNF-alpha response
    • Description: A sigE mutant showed a significantly higher and constant expression of the gene encoding TNF-alpha as compared to mice infected with the parental strain. Vaccination with the mutant significantly up-regulated TNF-alpha in mice lungs on days 1, 21, and 28 post-infection as compared to infection with the wild type (Hernandez et al., 2010).
    • Detailed Gene Information: Click Here.
  • Information about this animal model: Mouse Model for TB research

Human Response

  • Description: The human monocytic cell line THP-1 was obtained from the ATCC, and cultures were grown (Fontán et al., 2008).
  • Host APOBEC3B response
    • Description: A sigE mutant upregulates APOBEC3B in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host C19orf66 response
    • Description: A sigE mutant upregulates C19orf66 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host CD70 response
    • Description: A sigE mutant upregulates CD70 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host GPR182 response
    • Description: A sigE mutant upregulates GPR182 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host HIST1H2BE response
    • Description: A sigE mutant upregulates HIST1H2BE in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host HIST2H4A response
    • Description: A sigE mutant upregulates HIST2H4A in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host PCDHB1 response
    • Description: A sigE mutant upregulates PCDHB1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host PTCD1 response
    • Description: A sigE mutant upregulates PTCD1 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host STAG3 response
    • Description: A sigE mutant upregulates STAG3 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host TBX21 response
    • Description: A sigE mutant upregulates TBX21 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
  • Host TSC22D4 response
    • Description: A sigE mutant upregulates TSC22D4 in human THP-1 cells, which was not significantly upregulated after infection with the wild type pathogen (Fontán et al., 2008).
    • Detailed Gene Information: Click Here.
References
Fontán et al., 2008: Fontán PA, Aris V, Alvarez ME, Ghanny S, Cheng J, Soteropoulos P, Trevani A, Pine R, Smith I. Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response. The Journal of infectious diseases. 2008; 198(6); 877-885. [PubMed: 18657035].
Hernandez et al., 2010: Hernandez Pando R, Aguilar LD, Smith I, Manganelli R. Immunogenicity and protection induced by a Mycobacterium tuberculosis sigE mutant in a BALB/c mouse model of progressive pulmonary tuberculosis. Infection and immunity. 2010; 78(7); 3168-3176. [PubMed: 20457786].