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Vaccine Detail

Mycobacterium avium relA mutant vaccine
Vaccine Information
  • Vaccine Name: Mycobacterium avium relA mutant vaccine
  • Target Pathogen: Mycobacterium avium
  • Vaccine Ontology ID: VO_0002779
  • Type: Live, attenuated vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Goat
  • relA gene engineering:
    • Type: Gene mutation
    • Description: This relA mutant is from Mycobacterium avium (Park et al., 2011).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Oral immunization
Host Response

Goat Response

  • Persistence: A relA mutant is attenuated in goats (Park et al., 2011).
  • Efficacy: A relA mutant induces protection in goats from challenge with wild type M. avium (Park et al., 2011).
  • Host IFNG response
    • Description: Expression of IFN-gamma was increased. Specifically, a quantitative RT-PCR revealed the gene encoding IFN-γ was up-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
    • Detailed Gene Information: Click Here.
  • Host IL10 response
    • Description: Expression of IL-10 was decreased. Specifically, a quantitative RT-PCR revealed the gene encoding IL-10 was down-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
    • Detailed Gene Information: Click Here.
  • Host IL17A response
    • Description: Expression of IL-17 was increased. Specifically, a quantitative RT-PCR revealed the gene encoding IL-17 was up-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
    • Detailed Gene Information: Click Here.
  • Host IL22 response
    • Description: Expression of IL-22 was increased (Park et al., 2011).
    • Detailed Gene Information: Click Here.

Cattle Response

  • Description: Cattle peripheral blood mononuclear cells (PBMC) were used for cytokine detection (Park et al., 2011).
  • Host IFNG response
    • Description: Expression of IFN-gamma was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IFN-γ was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
    • Detailed Gene Information: Click Here.
  • Host IL17A response
    • Description: Expression of IL-17 was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IL-17 was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
    • Detailed Gene Information: Click Here.
  • Host IL22 response
    • Description: Expression of IL-22 was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IL-22 was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
    • Detailed Gene Information: Click Here.
References
Park et al., 2011: Park KT, Allen AJ, Bannantine JP, Seo KS, Hamilton MJ, Abdellrazeq GS, Rihan HM, Grimm A, Davis WC. Evaluation of two mutants of Mycobacterium avium subsp. paratuberculosis as candidates for a live attenuated vaccine for Johne's disease. Vaccine. 2011; ; . [PubMed: 21565243].