• Vaccine Name: Mycobacterium avium relA mutant vaccine
• Target Pathogen: Mycobacterium avium
• Vaccine Ontology ID: VO_0002779
• Type: Live, attenuated vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Goat
• relA gene engineering:
  • Type: Gene mutation
  • Description: This relA mutant is from Mycobacterium avium (Park et al., 2011).
  • Detailed Gene Information: Click Here.
• Immunization Route: Oral immunization

Goat Response

• Persistence: A relA mutant is attenuated in goats (Park et al., 2011).
• Efficacy: A relA mutant induces protection in goats from challenge with wild type M. avium (Park et al., 2011).
• Host IFNG response
  • Description: Expression of IFN-gamma was increased. Specifically, a quantitative RT-PCR revealed the gene encoding IFN-γ was up-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
  • Detailed Gene Information: Click Here.
• Host IL10 response
  • Description: Expression of IL-10 was decreased. Specifically, a quantitative RT-PCR revealed the gene encoding IL-10 was down-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
  • Detailed Gene Information: Click Here.
• Host IL17A response
  • Description: Expression of IL-17 was increased. Specifically, a quantitative RT-PCR revealed the gene encoding IL-17 was up-regulated in PBMC stimulated with live Map (a M. avium relA mutant) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
  • Detailed Gene Information: Click Here.
• Host IL22 response
  • Description: Expression of IL-22 was increased (Park et al., 2011).
  • Detailed Gene Information: Click Here.

Cattle Response

• Description: Cattle peripheral blood mononuclear cells (PBMC) were used for cytokine detection (Park et al., 2011).
• Host IFNG response
  • Description: Expression of IFN-gamma was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IFN-γ was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
  • Detailed Gene Information: Click Here.
• Host IL17A response
  • Description: Expression of IL-17 was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IL-17 was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
  • Detailed Gene Information: Click Here.
• Host IL22 response
  • Description: Expression of IL-22 was increased in cattle PBMC. Specifically, a quantitative RT-PCR revealed the gene encoding IL-22 was up-regulated in PBMC stimulated with live Map (WT) three months post infection compared to the response of PBMC pre-infection (Park et al., 2011).
  • Detailed Gene Information: Click Here.