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Vaccine Detail

Francisella tularensis guaB mutant vaccine
Vaccine Information
  • Vaccine Name: Francisella tularensis guaB mutant vaccine
  • Target Pathogen: Francisella tularensis
  • Target Disease: Tularemia
  • Vaccine Ontology ID: VO_0002846
  • Type: Live, attenuated vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • guaB gene engineering:
    • Type: Gene mutation
    • Description: This guaB mutant is from Francisella tularensis (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Persistence: A guaB mutant is attenuated in mice (Santiago et al., 2009).
  • Efficacy: A guaB mutant induces significant protection in mice from challenge with wild type F. tularensis (Santiago et al., 2009).
  • Host Ccl5 response
    • Description: RANTES (Ccl5) and iNOS were induced after 4 hours of infection of the mutant in mouse macrophages and were significantly higher than media controls. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host Cxcl1 response
    • Description: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC (i.e., Cxcl1), and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host IL-1b response
    • Description: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host Il12a response
    • Description: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host Il12b response
    • Description: IL-12 p40 mRNA was induced later in mouse macrophages, but was expressed for a longer period of time than other expressed cytokines. Levels were similar to or greater than levels induced by WT LVS and were significantly greater than media controls (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host Nos2 response
    • Description: RANTES and iNOS were induced after 4 hours of infection of the mutant in mouse macrophages and were significantly higher than media controls. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host TNF-alpha response
    • Description: LVSΔguaB caused a rapid increase in the levels of transcription of TLR2-dependent cytokines including TNF-α, IL-1β, KC, and IL-12 p35 following infection of primary mouse macrophages as compared to media. Levels were similar to or greater than levels induced by WT LVS (Santiago et al., 2009).
    • Detailed Gene Information: Click Here.
References
Santiago et al., 2009: Santiago AE, Cole LE, Franco A, Vogel SN, Levine MM, Barry EM. Characterization of rationally attenuated Francisella tularensis vaccine strains that harbor deletions in the guaA and guaB genes. Vaccine. 2009; 27(18); 2426-2436. [PubMed: 19368784].