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Vaccine Detail

M2 mutant from Influenza A
Vaccine Information
  • Vaccine Name: M2 mutant from Influenza A
  • Target Pathogen: Influenza virus
  • Target Disease: Influenza (flu)
  • Product Name: M2KO
  • Vaccine Ontology ID: VO_0004287
  • Type: Live, attenuated vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • M2 from Influenza A virus (A/Puerto Rico/8/34(H1N1)) gene engineering:
    • Type: Gene mutation
    • Description: This M2 gene is from Influenza A (Watanabe et al., 2009).
    • Detailed Gene Information: Click Here.
  • Immunization Route: intranasal immunization
Host Response

Mouse Response

  • Vaccination Protocol: Intranasal vaccination of 4-week-old female BALB/c mice with different doses of M2KO virus (Watanabe et al., 2009).
  • Persistence: In nasal turbinates, no virus was recovered from any mice inoculated with the M2KO virus on days 3 and 6 post infection. Mice infected with 3 × 106 PFU of M2KO virus showed no body weight loss. Thus, the M2KO virus is attenuated (Watanabe et al., 2009).
  • Efficacy: Mice intranasally inoculated with M2KO virus, formalin-inactivated PR8, or PBS were challenged with a lethal dose of PR8 at 4 and 12 weeks postimmunization. We could not detect virus in organs of mice inoculated either with 3 × 106 or 3 × 105 PFU of M2KO viruses, indicating that vaccination with these amounts of M2KO virus gave mice sterile immunity (Watanabe et al., 2009).
  • Host IgA response
    • Description: IgG and IgA titers were determined by examination of sera, trachea/lungs, and nasal washes. Mice inoculated with M2KO had significantly higher IgG and IgA antibodies as compared to PBS inoculated mice. Antibody responses correlated with dosage of M2KO. Samples were obtained 4 weeks post-immunization (Watanabe et al., 2009).
    • Detailed Gene Information: Click Here.
  • Host IgG response
    • Description: IgG and IgA titers were determined by examination of sera, trachea/lungs, and nasal washes. Mice inoculated with M2KO had significantly higher IgG and IgA antibodies as compared to PBS inoculated mice. Antibody responses correlated with dosage of M2KO. Samples were obtained 4 weeks post-immunization (Watanabe et al., 2009).
    • Detailed Gene Information: Click Here.
References
Watanabe et al., 2009: Watanabe S, Watanabe T, Kawaoka Y. Influenza A virus lacking M2 protein as a live attenuated vaccine. Journal of virology. 2009; 83(11); 5947-5950. [PubMed: 19321619].