• Vaccine Name: Hepatitis B DNA vaccine pRc/CMV-HBs(S) encoding HBsAg
• Target Pathogen: Hepatitis B virus
• Target Disease: Hepatitis B
• Vaccine Ontology ID: VO_0004354
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• HbS gene engineering:
  • Type: DNA vaccine construction
  • Description: This DNA vaccine expressed the HBV surface antigen (HBsAg) (Khatri et al., 2008).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005047
• Immunization Route: intranasal immunization

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: When mice are immunized with recombinant HBsAg, the main type of immune response generated is the antibody response. However, a Th1/CTL response was also elicited, which is important to facilitate eradication of HBV infection and can be utilized for therapeutic immunization of HBV chronic carriers (Khatri et al., 2008).
• Efficacy: Nasal administration of nanoparticles resulted in serum anti-HBsAg titre that was less compared to that elicited by naked DNA and alum adsorbed HBsAg, but the mice were seroprotective within 2 weeks and the immunoglobulin level was above the clinically protective level (>10 mIU/ml) suggesting successful generation of systemic immunity. Levels of 1 and 10 mIU/ml are well-established standards for anti-HBs antibody levels in mice and humans, respectively and are considered sufficient to confer protection against the disease (Khatri et al., 2008).