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Vaccine Detail

M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A
Vaccine Information
  • Vaccine Name: M. tuberculosis DNA vaccine pcDNA3.1+/Ag85A DNA encoding Ag85A
  • Target Pathogen: Mycobacterium tuberculosis
  • Target Disease: Tuberculosis
  • Vaccine Ontology ID: VO_0004356
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • FbpA (Ag85A) gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pcDNA3.1 expressed Mycobacterium antigen 85A (Ag85A) (Wang et al., 2010).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Orally administrated liposomal-pcDNA3.1+/Ag85A DNA was efficiently incorporated into mucosal epithelium of the small intestine, Peyer's patches (PP). This initiated a Ag85A-specific Th1 dominant immune response, as evidenced by increased secretion of IL-2, IFN-γ, and no change of IL-4 (Wang et al., 2010)
  • Efficacy: Liposomal-pcDNA3.1+/Ag85A DNA significantly increased the protection by giving a markedly reduction of TB burden in the lung, demonstrating that the TB-specific immune responses elicited by oral administration of liposomal-pcDNA3.1+/Ag85A DNA led to an enhanced host defense against TB infection. (Wang et al., 2010)
  • Information about this animal model: Mouse Model for TB research
References
Wang et al., 2010: Wang D, Xu J, Feng Y, Liu Y, Mchenga SS, Shan F, Sasaki J, Lu C. Liposomal oral DNA vaccine (mycobacterium DNA) elicits immune response. Vaccine. 2010; 28(18); 3134-3142. [PubMed: 20197133].