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Vaccine Detail

T. cruzi DNA vaccine pUB-ASP-2
Vaccine Information
  • Vaccine Name: T. cruzi DNA vaccine pUB-ASP-2
  • Target Pathogen: Trypanosoma cruzi
  • Target Disease: Chagas disease
  • Vaccine Ontology ID: VO_0004376
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • amastigote surface protein-2 gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pcDNA3.1 expressed amastigote surface protein 2 (ASP-2 ) (Chou et al., 2010).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: The absolute number of both IFN-γ+CD8+ T cells and GZM-b+CD8+ T cells in the spleen was significantly higher in pUB-ASP-2 immunized mice. Immunization with pUB-ASP-2 promotes CD8+ T cell activation, and enhances the expression level of IFN-γ and GZM-b in CD8+ T cells (Chou et al., 2010).
  • Efficacy: After being challenged with T. cruzi, mice immunized with pUB-ASP-2 developed a lower parasitemia than control pcDNA immunized groups; survival was also prolonged by immunization with pUB-ASP-2. Six out of seven pUB-ASP-2 immunized mice survived until the end of the experiment (Chou et al., 2010).
References
Chou et al., 2010: Chou B, Hiromatsu K, Hisaeda H, Duan X, Imai T, Murata S, Tanaka K, Himeno K. Genetic immunization based on the ubiquitin-fusion degradation pathway against Trypanosoma cruzi. Biochemical and biophysical research communications. 2010; 392(3); 277-282. [PubMed: 20059980].