• Vaccine Name: Autoimmune diabetes DNA vaccine pND2-GAD65-BAX encoding GAD and BAX
• Target Pathogen: Diabetes
• Target Disease: Diabetes
• Vaccine Ontology ID: VO_0004405
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• BAX gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pND2 expressed secreted BAX (Li et al., 2004).
  • Detailed Gene Information: Click Here.
• GAD65 gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pND2 expressed secreted GAD (Li et al., 2004).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000293
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0003057
• Efficacy: Data revealed that the only mice showing a significant decrease in diabetes onset were those receiving plasmid pND2-SGAD55-BAX (47% diabetic) compared to mice receiving plasmid pND2-GAD65 (93% diabetic), pND2-GAD65-BAX (87% diabetic), and untreated mice (93% diabetic). Insulitis scoring showed that the decrease in diabetes incidence observed with mice receiving plasmid pND2-SGAD55-BAX was associated with a significant decrease in islet inflammation when compared to untreated mice, and mice receiving either plasmid pND2, pND2-SRUC3, -GAD65-BAX, or -GAD65 (Li et al., 2004).