• Vaccine Name: Insulin-dependent diabetes mellitus DNA vaccine JwGAD65 + IL-4
• Target Pathogen: Diabetes
• Target Disease: Diabetes
• Vaccine Ontology ID: VO_0004406
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• Antigen: a fusion protein consisting of a portion of murine GAD65 and IgGFc (Tisch et al., 2001)
• Gad65 gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector Jw4303 expressed a secreted fusion protein consisting of a fragment of glutamic acid decarboxylase 65 (GAD65) linked to IgGFc (Tisch et al., 2001).
  • Detailed Gene Information: Click Here.
• IgG Fc gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000276
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0003057
• Efficacy: After monitoring animals up to 58 wk of age, the majority of unimmunized nonobese diabetic (NOD) mice (70%) or animals immunized with JwHEL + IL-4 (75%) or JwGAD65 (83%) had developed diabetes. In contrast, only 17% of NOD mice immunized with JwGAD65 + IL-4 developed diabetes (p = 0.03 vs unimmunized, p = 0.01 vs JwHEL + IL-4, χ2 test) (Tisch et al., 2001).