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Vaccine Detail

Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65
Vaccine Information
  • Vaccine Name: Adjuvant arthritis DNA vaccine pCMV3.65 encoding HSP65
  • Target Pathogen: Arthritis
  • Vaccine Ontology ID: VO_0004407
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Rat
  • hsp65 gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pcDNA3 expressed mycobacterial heat shock protein 65 (hsp65) (Ragno et al., 1997).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Rat Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Rats treated with pCMV3.65 showed a significantly elevated antibody levels (median 0.707) when compared with vector-treated rats (median 0.351; P < 0.01) and with naive animals (median 0.0715; P < 0.01). Similarly, spleen cells from pCMV3.65-treated rats exhibited significantly higher proliferative responses against the mycobacterial recombinant antigen when compared with vector treated animals (P < 0.05) or with naive rats (P < 0.01) (Ragno et al., 1997).
  • Efficacy: The pCMV3.65-treated rats were significantly protected from disease development in comparison with the control groups injected with the empty plasmid (Ragno et al., 1997).
References
Ragno et al., 1997: Ragno S, Colston MJ, Lowrie DB, Winrow VR, Blake DR, Tascon R. Protection of rats from adjuvant arthritis by immunization with naked DNA encoding for mycobacterial heat shock protein 65. Arthritis and rheumatism. 1997; 40(2); 277-283. [PubMed: 9041939].