• Vaccine Name: IHNV DNA vaccine pCDNA-G encoding G
• Target Pathogen: Infectious Hematopoietic Necrosis Virus
• Target Disease: Infectious hematopoietic necrosis
• Vaccine Ontology ID: VO_0004420
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Rainbow trout, salmon
• G glycoprotein gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCDNA3.1 expressed the glycoprotein (G) (Corbeil et al., 1999).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Intramuscular injection (i.m.)

Fish Response

• Vaccine Immune Response Type: VO_0000286
• Immune Response: The G protein expressed by the DNA vaccine construct did confer significant protection in fry up to 80 days post-immunization and induced protective neutralizing antibodies (Corbeil et al., 1999).
• Efficacy: Cumulative percent mortality in fish immunized with pCDNA-G ranged from 0 to 2% whereas mortality in fish immunized with DNA vaccines that contained other proteins or were left unhandled ranged from 36 to 76%. Significant protection was elicited up to 80 days post-immunization, and the vaccine also induced protective neutralizing antibodies (Corbeil et al., 1999).