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Vaccine Detail

Melanoma DNA vaccine pNL3-MAGE-1-GM
Vaccine Information
  • Vaccine Name: Melanoma DNA vaccine pNL3-MAGE-1-GM
  • Target Pathogen: Cancer
  • Target Disease: Cancer
  • Vaccine Ontology ID: VO_0004434
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • GM-CSF gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pNGVL3 expressed GM-CSF and MAGE-1 (Sun et al., 2002).
    • Detailed Gene Information: Click Here.
  • MAGE-1 gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pNGVL3 expressed GM-CSF and MAGE-1 (Sun et al., 2002).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pNGVL3 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: C57BL/6 mice were immunized on days 0, 7, and 17 with the different MAGE-1 plasmids. After 6 days, the mice were challenged with B16 melanoma cells that express the human MAGE-1 antigen. Mice immunized with plasmids co-expressing MAGE-1 and GM-CSF had significantly fewer tumor colonies on their lungs than mice from any of the other immunization groups. In fact, 60% of the mice immunized with DNA vaccines that co-express MAGE-1 and GM-CSF had fewer than five tumor colonies on their lungs, whereas this was true of a little less than 20% of the mice immunized with MAGE-1 alone vaccines or co-inoculation with MAGE-1 vaccines and GM-CSF expressing plasmid (Sun et al., 2002).
References
Sun et al., 2002: Sun X, Hodge LM, Jones HP, Tabor L, Simecka JW. Co-expression of granulocyte-macrophage colony-stimulating factor with antigen enhances humoral and tumor immunity after DNA vaccination. Vaccine. 2002; 20(9-10); 1466-1474. [PubMed: 11818167].