• Vaccine Name: Melanoma DNA vaccine pUB-TRP-2 encoding a fusion protein linking murine ubiquitin (UB) to the N-terminus of the full-length mTRP-2
• Target Pathogen: Cancer
• Target Disease: Cancer
• Vaccine Ontology ID: VO_0004443
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• Ubiquitin gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• Trp2 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000158
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: A week after the last immunization, mice were challenged subcutaneously with 2 105 B16F1 cells. Control mice and mice immunized with pTRP-2 showed rapid tumor growth, whereas 85% of mice immunized with pUB-TRP-2 were free of tumors and the remaining 15% of the mice exhibited almost complete suppression of tumor growth. Strictly, all mice immunized with pUB-TRP-2 survived over 80 days after implantation of tumor cells, although all mice of the other two groups died within 60 days. Furthermore, immunization with pUB-TRP-2 was also effective in suppressing the growth of B16F10 melanoma cells, a more virulent type of melanoma (Zhang et al., 2005).