• Vaccine Name: PRRSV DNA vaccine pCA-U-optiGP5
• Target Pathogen: Porcine respiratory and reproductive syndrome virus
• Target Disease: Porcine Reproductive and Respiratory Syndrome (PRRS), Blue-Ear Pig Disease
• Vaccine Ontology ID: VO_0004506
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Pig
• ORF5 gene engineering:
  • Type: DNA vaccine construction
  • Description: Vector pCAGGS expressed the U-optiGP5 fusion gene was generated by linking optiGP5 downstream of ubiquitin. The Kozak sequence, GCCACC, served as the upstream start codon for all three fragments (Hou et al., 2008).
  • Detailed Gene Information: Click Here.
• ubiquitin gene engineering:
  • Type: DNA vaccine construction
  • Description: This vaccine expressed a sequence encoding pig ubiquitin that had its Gly76 changed to Ala76 (Hou et al., 2008).
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000099
• Immunization Route: Intramuscular injection (i.m.)

Pig Response

• Vaccine Immune Response Type: VO_0000286
• Efficacy: Four of six pigs in the pCA-U-optiGP5 group were devoid of visible pathological changes that were present in other vaccinated and control animals after challenge (Hou et al., 2008).