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Vaccine Detail

PRRSV DNA vaccine pCA-U-optiGP5
Vaccine Information
  • Vaccine Name: PRRSV DNA vaccine pCA-U-optiGP5
  • Target Pathogen: Porcine respiratory and reproductive syndrome virus
  • Target Disease: Porcine Reproductive and Respiratory Syndrome (PRRS), Blue-Ear Pig Disease
  • Vaccine Ontology ID: VO_0004506
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Pig
  • ORF5 gene engineering:
    • Type: DNA vaccine construction
    • Description: Vector pCAGGS expressed the U-optiGP5 fusion gene was generated by linking optiGP5 downstream of ubiquitin. The Kozak sequence, GCCACC, served as the upstream start codon for all three fragments (Hou et al., 2008).
    • Detailed Gene Information: Click Here.
  • ubiquitin gene engineering:
    • Type: DNA vaccine construction
    • Description: This vaccine expressed a sequence encoding pig ubiquitin that had its Gly76 changed to Ala76 (Hou et al., 2008).
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: Pcaggs DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Pig Response

  • Vaccine Immune Response Type: VO_0000286
  • Efficacy: Four of six pigs in the pCA-U-optiGP5 group were devoid of visible pathological changes that were present in other vaccinated and control animals after challenge (Hou et al., 2008).
References
Hou et al., 2008: Hou YH, Chen J, Tong GZ, Tian ZJ, Zhou YJ, Li GX, Li X, Peng JM, An TQ, Yang HC. A recombinant plasmid co-expressing swine ubiquitin and the GP5 encoding-gene of porcine reproductive and respiratory syndrome virus induces protective immunity in piglets. Vaccine. 2008; 26(11); 1438-1449. [PubMed: 18262692].