• Vaccine Name: B. burgdorferi DNA vaccine encoding strain B31 OspA
• Target Pathogen: Borrelia burgdorferi
• Target Disease: Lyme Disease
• Vaccine Ontology ID: VO_0004513
• Type: DNA vaccine
• Status: Research
• OspA gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005023
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Female C3H/HeN mice (6-10 weeks old) were injected with plasmid diluted in sterile 0.9% saline. Each mouse received intramuscular injections into left and right rectus femoris muscles with 50 J-lg of plasmid in 50 J-lL of saline. For immunization with protein, mice were injected in the back with purified B31 rOspA lipoprotein (Luke et al., 1997).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: Sera from mice immunized with the ospA-bearing plasmid DNA had high levels of antibody that recognized both native and rOspA lipoprotein (Luke et al., 1997).
• Challenge Protocol: Mice were challenged intradermally by injecting 100 x ID50 of the infectious isolate at the base of the tail (Luke et al., 1997).
• Efficacy: Immunization with VR2210 protected 100% of mice against infection with Sh-2-82 or N40, 2 infectious strains of B. burgdorferi (Luke et al., 1997).