• Vaccine Name: Herpes Simplex Virus 2 DNA Vaccine encoding gD
• Target Pathogen: Herpes simplex virus type 1 and 2
• Target Disease: Herpes
• Vaccine Ontology ID: VO_0004522
• Type: DNA vaccine
• Status: Research
• gD gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005074
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: gD DNA vaccine or empty plasmid was administered intramuscularly on days 0 and 2. gD-liposomes or empty liposomes were administered 3 weeks after the DNA prime (50 μl total dose per mouse per time point in both nostrils) (Tirabassi et al., 2011).
• Vaccine Immune Response Type: VO_0000286
• Immune Response: The vaccine stimulated high titers of serum neutralising antibodies, a DNA priming dose dependent T helper type response, enhanced mucosal immune responses and potent protective immunity at the vaginal cavity, the portal of entry for the virus (Tirabassi et al., 2011).
• Challenge Protocol: The clinical isolate, HSV-2 strain MS was grown and titered in Vero cells. LD50 was titrated in Balb/c mice prior to the challenge experiments. Five days prior to infection, mice were injected subcutaneously with 2 mg of medroxyprogesterone. On the day of infection, animals were anesthetised intraperitoneally with a ketamine/xylazine mixture and instilled intravaginally with a 20 μl suspension containing the indicated virus dose (Tirabassi et al., 2011).
• Efficacy: The vaccine induced durable protection in mice, demonstrated by a 60% survival rate when lethal infections were performed 20 weeks after the immunisation primed with 0.5 μg of DNA vaccine (Tirabassi et al., 2011).