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Vaccine Detail

T. gondii DNA vaccine ROP8-pVAX-1
Vaccine Information
  • Vaccine Name: T. gondii DNA vaccine ROP8-pVAX-1
  • Target Pathogen: Toxoplasma gondii
  • Target Disease: Toxoplasmosis
  • Vaccine Ontology ID: VO_0004531
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • ROP8 gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pVAX1 DNA vaccine plasmid
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: splenocytes from mice immunized with ROP8-pVAX-1 secreted significantly high levels of IFN-γ (816 ± 26.3 pg/mL) compared with spleen cells cultured from mice immunized with pVAX-1 (48 ± 10.8) or PBS (45 ± 6.6). Only a low level of IL-4 was detected in the culture supernatant of splenocytes from mice immunized with ROP8-pVAX-1 (148 ± 18.3) but was significantly higher compared with mice immunized with pVAX-1 (50 ± 13.6) or PBS (47 ± 6.1) (Parthasarathy et al., 2013).
  • Efficacy: Mice immunized with ROP8-pVAX-1 DNA had a 100% survival rate until Day 9, whereas all the control mice died. On top of that, mice immunized with ROP8-pVAX-1 DNA showed a significant increase in the survival time (29 days, P < 0.05) (Parthasarathy et al., 2013).
References
Parthasarathy et al., 2013: Parthasarathy S, Fong MY, Ramaswamy K, Lau YL. Protective Immune Response in BALB/c Mice Induced by DNA Vaccine of the ROP8 gene of Toxoplasma gondii. The American journal of tropical medicine and hygiene. 2013; 88(5); 883-887. [PubMed: 23509124].