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Vaccine Detail

S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
Vaccine Information
  • Vaccine Name: S. aureus DNA vaccine encoding Efb, FnbpA, ClfA, Cna
  • Target Pathogen: Staphylococcus aureus
  • Target Disease: Staph infections
  • Vaccine Ontology ID: VO_0004542
  • Type: DNA vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • Antigen: fibrinogen binding protein (Efb), fibronectin-binding protein A (FnbpA), clumping factor A (ClfA) and collagen-binding protein (Cna) from S. aureus strain 1706 (Castagliuolo et al., 2006)
  • ClfA gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • cna gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • efb gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • fnbA gene engineering:
    • Type: DNA vaccine construction
    • Description:
    • Detailed Gene Information: Click Here.
  • DNA vaccine plasmid: pcDNA3.1 DNA vaccine plasmid
  • Immunization Route: intranasal immunization
Host Response

Mouse Response

  • Vaccine Immune Response Type: VO_0000286
  • Immune Response: Intranasal immunization with a pDNA mixture coding the four adhesins triggered significant levels of specific serum and mucosal Ig that inhibited S. aureus adhesion to cow mammary gland epithelial cells in vitro. Splenocytes of immunized mice challenged in vitro with S. aureus extracts showed a strong proliferative response (Castagliuolo et al., 2006).
  • Efficacy: In non immune mice or mice injected with empty vectors, a large number of bacteria was recovered from the mammary glands. Whereas, in mice immunized with the genetic vaccine coding the four S. aureus adhesins, the amount of bacteria recovered from the mammary glands was significantly decreased (Castagliuolo et al., 2006).
References
Castagliuolo et al., 2006: Castagliuolo I, Piccinini R, Beggiao E, Palù G, Mengoli C, Ditadi F, Vicenzoni G, Zecconi A. Mucosal genetic immunization against four adhesins protects against Staphylococcus aureus-induced mastitis in mice. Vaccine. 2006; 24(20); 4393-4402. [PubMed: 16580097].