• Vaccine Name: Dengue Virus DNA Vaccine encoding NS1
• Target Pathogen: Dengue Virus
• Target Disease: Dengue Fever
• Vaccine Ontology ID: VO_0004549
• Type: DNA vaccine
• Status: Research
• Antigen: NS1 from dengue virus 2 strain NGC (Costa et al., 2006)
• NS1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0005035
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Male Balb/c mice, 4–6 weeks old, were intramuscularly injected in each hind limb tibialis anterior muscle with 50 μg of the pcTPANS1 or pcDNA3 plasmids dissolved in 50 μl of PBS (100 μg/dose/mice). Mice were inoculated with two DNA doses, given two weeks apart, and bled before immunization and 4 weeks after the first DNA dose (Costa et al., 2006).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: All Balb/c mice intramuscularly inoculated with the pcTPANS1 presented high levels of NS1-specifc antibodies (Costa et al., 2006).
• Challenge Protocol: Two weeks after the second DNA dose, each immunized mouse was challenged with intracerebral inoculation with 30 μL of 4.32 log10 PFU of DENV2 (Costa et al., 2006).
• Efficacy: Vaccinated animals were challenged with intracerebral DENV-2 virus inoculations and a 100% survival was observed (Costa et al., 2006).