• Vaccine Name: Influenza virus DNA vaccine M2-DNA/M2-Ad
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004568
• Type: DNA vaccine
• Status: Research
• Host Species as Laboratory Animal Model: Mouse
• M2 from Influenza A virus (A/Puerto Rico/8/34(H1N1)) gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0000334
• Immunization Route: Intramuscular injection (i.m.)

Baboon Response

• Vaccination Protocol: DNA vaccination at doses of 50 μg/mouse in low-endotoxin PBS was given intramuscularly in the quadriceps, half to each leg, in 3 doses 2 weeks apart. In some experiments, mice were given a booster injection of rAd intramuscularly at a dose of 1010 particles/mouse, 2–3 weeks after the last dose of DNA (Tompkins et al., 2007).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and induced T-cell responses (Tompkins et al., 2007).
• Challenge Protocol: Challenge virus in 50 μL of PBS was administered intranasally to anesthetized mice. Isoflurane or ketamine/xylazine was used for mice challenged with H1N1 subtype. Subtype H5N1 was administered intranasally to mice anesthetized with 2,2,2-tribromoethanol in tert-amyl alcohol (Tompkins et al., 2007).
• Efficacy: Of mice vaccinated with M2-DNA, 100% survived challenge with A/PR/8 and A/FM virus, both subtypes of the H1N1 virus (Tompkins et al., 2007).