• Vaccine Name: Japanese encephalitis virus DNA Vaccine P-JEV
• Target Pathogen: Japanese encephalitis virus
• Target Disease: Japanese encephalitis
• Vaccine Ontology ID: VO_0004586
• Type: DNA vaccine
• Status: Research
• E gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• NS1 gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• M protein gene engineering:
  • Type: DNA vaccine construction
  • Description:
  • Detailed Gene Information: Click Here.
• DNA vaccine plasmid:
  • DNA vaccine plasmid name:
  • DNA vaccine plasmid VO ID: VO_0001004
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Control mice received 1 μg of pcDNA3.1/V5-His plasmid per dose. While same amount of recombinant plasmid, pcDNA3.1/V5-His-P-JEV was used to immunize the experimental group. Groups of BALB/c mice (n = 10), received two booster doses with equal amount of DNA after every 2 weeks (Kulkarni et al., 2012).
• Vaccine Immune Response Type: VO_0003057
• Immune Response: Anti-JEV antibodies were detectable in all mouse groups 31 days after immunization. Levels of these antibodies increased after the first booster immunization and were further enhanced after the second booster dose. Following the challenge, pcDNA3.1/V5-His-P-JEV immunized mice showed significantly enhanced JEV antibody titres (Kulkarni et al., 2012).
• Challenge Protocol: At 6 weeks after immunization, mice were challenged with lethal dose of 100LD50 with JEV 733913 strain by intraperitonial route, followed by 1% starch by the intracerebral route to breach the blood brain barrier (Kulkarni et al., 2012).
• Efficacy: Approximately 83% of mice immunized with the vaccine survived the challenge injection (Kulkarni et al., 2012).