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Vaccine Detail

S. pneumoniae pspA-rBCG Vector Vaccine
Vaccine Information
  • Vaccine Name: S. pneumoniae pspA-rBCG Vector Vaccine
  • Target Pathogen: Streptococcus pneumoniae
  • Target Disease: Pneumonia
  • Vaccine Ontology ID: VO_0004601
  • Type: Recombinant vector vaccine
  • Status: Research
  • Host Species as Laboratory Animal Model: Mouse
  • Antigen: Pneumococcal surface protein A (PspA) present on all strains of pneumococci (Langermann et al., 1994)
  • PspA gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Vector: BCG vaccine vector
  • Immunization Route: Intramuscular injection (i.m.)
  • Description: Streptococcus pneumoniae is one of the leading causes of bacterial pneumonia and acute otitis media, a type of meningitis. It is becoming increasingly antibiotic resistant and the current available vaccine does not elicit protection in children. (Langermann et al., 1994)
Host Response

Mouse Response

  • Host Strain: BALB/c and C3H/HeJ mice
  • Vaccination Protocol: A single intraperitoneal inoculation with a live BCG bacterial vaccine expressing PspA as a secreted mature protein in or a chimeric exported lipoprotein (rBCG-PspA), followed by a booster at 17 weeks(Langermann et al., 1994)
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: BALB/c and C3H/HeJ mice immunized with rBCG-PspA vaccine strains were challenged intraperitoneally with 104 CFU of the highly lethal S. pneumoniae strain WU2 (Langermann et al., 1994)
  • Challenge Detail:
    No. Pathogen Name Dose Route Age Interval
    1 S. pneumoniae strain WU2 10000 CFU in volume ml Intraperitoneal injection (i.p.) day day
  • Efficacy: Mice experienced passive protection with the use of rBCG against lethal challenge with the highly virulent, encapsulated WU2 strain of Streptococcus pneumoniae and two additional virulent strains exhibiting heterologous PspA and capsular sero- types(Langermann et al., 1994)
References
Langermann et al., 1994: Langermann S, Palaszynski SR, Burlein JE, Koenig S, Hanson MS, Briles DE, Stover CK. Protective humoral response against pneumococcal infection in mice elicited by recombinant bacille Calmette-Guérin vaccines expressing pneumococcal surface protein A. The Journal of experimental medicine. 1994; 180(6); 2277-2286. [PubMed: 7964500].