• Vaccine Name: RB51--MIC3/GRA2/GRA6/MIC1/SRS2
• Target Pathogen: Neospora caninum
• Target Disease: Neosporosis
• Vaccine Ontology ID: VO_0004649
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• MIC1 gene engineering:
  • Type: Recombinant vector construction
  • Description: Neospora caninum protective antigens MIC1, MIC3, GRA2, GRA6 and RS2 were expressed in strain RB51 (Ramamoorthy et al., 2007).
  • Detailed Gene Information: Click Here.
• SRS2 gene engineering:
  • Type: Recombinant vector construction
  • Description: Neospora caninum protective antigens MIC1, MIC3, GRA2, GRA6 and RS2 were expressed in strain RB51 (Ramamoorthy et al., 2007).
  • Detailed Gene Information: Click Here.
• GRA6 gene engineering:
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click Here.
• Vector:
  • Vector name:
  • VO vector ID: VO_0000020
• Preparation: A recombinant strain RB51 expressing N. caninum antigen. Neospora caninum protective antigens MIC1, MIC3, GRA2, GRA6 and SRS2 were expressed in strain RB51 (Ramamoorthy et al., 2007).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Female C57BL/6 mice were vaccinated with a recombinant strain RB51 expressing N. caninum antigen or irradiated tachyzoites, boosted 4 weeks later and then bred (Ramamoorthy et al., 2007).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: Vaccinated mice were challenged with 5 x 10^6 N caninum tachyzoites between days 11-13 of pregnancy (Ramamoorthy et al., 2007).
• Efficacy: The RB51-MIC3, RB51-GRA6, irradiated tachyzoite vaccine, pooled strain RB51-Neospora vaccine, RB51-MIC1 and RB51-SRS2 vaccines elicited approximately 6-38% protection against vertical transmission. B. abortus strain RB51 expressing the specific N. caninum antigens induced substantial protection against vertical transmission of N. caninum in mice (Ramamoorthy et al., 2007).