• Vaccine Name: rLAG- PA-DCpep
• Target Pathogen: Bacillus anthracis
• Target Disease: Anthrax
• Vaccine Ontology ID: VO_0004715
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Mouse
• pagA gene engineering:
  • Type: Recombinant vector construction
  • Description:
  • Detailed Gene Information: Click Here.
• Vector:
  • Vector name:
  • VO vector ID: VO_0001066
• Preparation: Targeted B. anthracis protective antigen (PA) genetically fused to a DC-binding peptide (DCpep) was delivered by Lactobacillus acidophilus (Mohamadzadeh et al., 2010)
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: Groups of mice were orally vaccinated with 100 µl (108 CFU) L. gasseri expressing PA–DCpep, PA–Ctrlpep, or cells harboring the empty vector. Oral vaccination was administered four times on a weekly basis (Mohamadzadeh et al., 2010).
• Vaccine Immune Response Type: VO_0003057
• Challenge Protocol: The groups of mice were challenged intraperitoneally with B. anthracis Sterne pXO1+/pXO2- (5 × 10^4 CFU/mouse) (Mohamadzadeh et al., 2010).
• Efficacy: L. gasseri expressing PA–DCpep fusion was 100% efficacious in protection of the mice compared with 30% survival when vaccinated with L. gasseri expressing PA–Ctrl pep (Figure 3A & B). Additionally, vaccinated mice with recombinant PA plus alhydrogel were fully protected from Sterne lethal challenge (Mohamadzadeh et al., 2010).