• Vaccine Name: rORFV-D1701-V-HAh5n (H5N1)
• Target Pathogen: Influenza virus
• Target Disease: Influenza (flu)
• Vaccine Ontology ID: VO_0004765
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Baboon
• Vector:
  • Vector name:
  • VO vector ID: VO_0001108
• Preparation: ORFV recombinants expressing the hemagglutinin (HA) or nucleoprotein (NP) of the highly pathogenic avian influenza virus (HPAIV) H5N1 (Rohde et al., 2013).
• Immunization Route: Intramuscular injection (i.m.)

Mouse Response

• Vaccination Protocol: The mice were immunized with varying doses of D1701-V-HAh5n (Rohde et al., 2013).
• Vaccine Immune Response Type: VO_0000286
• Challenge Protocol: Mice were instilled intranasally (i.n.) under anaesthesia [20] using 50 µl of the indicated mouse 50% lethal dose (MLD50) of HPAIV. For BALB/c mice 1× MLD50 corresponded to 7×10^1 plaque-forming units (pfu) of strain MB1, 2×10^1 pfu of strain SN1 and 1×10^4 pfu of strain PR8. For C57BL/6 mice 2×10^3 pfu of strain MB1, 1.4×103^ pfu of strain PR8 matched to 1× MLD50 (Rohde et al., 2013).
• Efficacy: Despite adequate expression of NP, the recombinant D1701-V-NPh5 completely failed to protect mice from lethal challenge. However, the H5 HA-expressing recombinant D1701-V-HAh5n mediated solid protection in a dose-dependent manner. Two intramuscular (i.m.) injections of the HA-expressing recombinant protected all animals from lethal HPAIV infection without loss of body weight. Notably, the immunized mice resisted cross-clade H5N1 and heterologous H1N1 (strain PR8) influenza virus challenge (Rohde et al., 2013).