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Vaccine Detail

rORFV-RHDV-VP60
Vaccine Information
  • Vaccine Name: rORFV-RHDV-VP60
  • Target Pathogen: Rabbit hemorrhagic disease virus
  • Target Disease: Rabbit hemorrhagic disease
  • Vaccine Ontology ID: VO_0004767
  • Type: Recombinant vector vaccine
  • Status: Research
  • Host Species for Licensed Use: Rabbit
  • ORF1 gene engineering:
    • Type: Recombinant protein preparation
    • Description: Recombinant Orf virus (ORFV; Parapoxvirus) expressing the major capsid protein VP1 (VP60) of the calicivirus, rabbit hemorrhagic disease virus (RHDV) (Rohde et al., 2011).
    • Detailed Gene Information: Click Here.
  • Vector: ORF virus vaccine vector
  • Preparation: (Rohde et al., 2011) Anew recombinant Orf virus (ORFV; Parapoxvirus) expressing the major capsid protein VP1 (VP60) of RHDV.
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Rabbit Response

  • Vaccination Protocol: The animals were immunized once, twice or 3-times by the intramuscular (i.m.) route with 1 ml D1701-V-VP1 containing 107 PFU (plaque-forming units), 106 PFU, or 105 PFU, respectively. For controls, groups of rabbits were vaccinated with the commercial RHDV vaccine “RIKA-VACC” (Riemser Arzneimittel AG, Germany) or remained non-vaccinated (Rohde et al., 2011).
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: Twenty two days after the last vaccination, the animals were inoculated i.m. with 1 ml containing 104 LD50 (lethal dose 50) of the virulent RHDV strain “Eisenhüttenstadt” (Rohde et al., 2011).
  • Efficacy: All animals immunized with the ORFV-vectored vaccine survived without any clinical signs, similar to animals control immunized with the commercially available inactivated RHDV vaccine RIKA-VACC. As expected, all non-immunized rabbits died within 48 h after challenge with RHD-specific clinical symptoms (Rohde et al., 2011).
References
Rohde et al., 2011: Rohde J, Schirrmeier H, Granzow H, Rziha HJ. A new recombinant Orf virus (ORFV, Parapoxvirus) protects rabbits against lethal infection with rabbit hemorrhagic disease virus (RHDV). Vaccine. 2011; 29(49); 9256-9264. [PubMed: 22001119].