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Vaccine Detail

rORFV-PRV-gC/gD
Vaccine Information
  • Vaccine Name: rORFV-PRV-gC/gD
  • Target Pathogen: Pseudorabies virus
  • Target Disease: Aujeszky's disease
  • Vaccine Ontology ID: VO_0004770
  • Type: Recombinant vector vaccine
  • Status: Research
  • Host Species for Licensed Use: Baboon
  • Vector: ORF virus vaccine vector
  • Preparation: Based on the attenuated ORFV strain D1701-V, recombinants were produced that express the glycoproteins gC (D1701-VrVgC) or gD (D1701-VrVgD) of the alphaherpesvirus of swine, pseudorabies virus (PRV) (Fischer et al., 2003).
  • Immunization Route: Intramuscular injection (i.m.)
Host Response

Mouse Response

  • Vaccination Protocol: Mice were injected intramuscularly (i.m.) with 107 TCID50s of the ORFV recombinants expressing the PRV glycoproteins in a total volume of 0.2 ml (0.1 ml for each hind leg). Immunization was repeated at 2-week intervals (Fischer et al., 2003).
  • Vaccine Immune Response Type: VO_0003057
  • Challenge Protocol: Mice were challenge infected intraperitoneally (i.p.) with 102 PFU of the highly virulent PRV strain NIA-3, and the C57BL/6 and 129/Sv/Ev mice were infected with 103 PFU
  • Efficacy: Single or combined immunization with the ORFV recombinants protected different mouse strains of a host species nonpermissive for ORFV against a fulminant, lethal PRV challenge infection equal to immunization with PRV live vaccine. Most notably, even a single immunization with D1701-VrVgC was protective, whereas two applications of D1701-VrVgD were required for immune protection (Fischer et al., 2003).
References
Fischer et al., 2003: Fischer T, Planz O, Stitz L, Rziha HJ. Novel recombinant parapoxvirus vectors induce protective humoral and cellular immunity against lethal herpesvirus challenge infection in mice. Journal of virology. 2003; 77(17); 9312-9323. [PubMed: 12915547].