• Vaccine Name: BL21(DE3)/pUTa-E + pET28a-FT
• Target Pathogen: Edwardsiella tarda
• Vaccine Ontology ID: VO_0004804
• Type: Recombinant vector vaccine
• Status: Research
• Host Species for Licensed Use: Zebrafish
• Vector:
  • Vector name:
  • VO vector ID: VO_0001070
• Preparation: Cell-penetrating peptides (CPPs) are short cationic/amphipathic peptides which facilitate cellular uptake of various molecular cargoes and therefore have great potentials in vector vaccine design. Among the tested CPPs, TAT showed an excellent capability to deliver the cargo protein EGFP into cytoplasm (Ma et al., 2014). In order to establish an efficient antigen delivery system in Escherichia coli, the EGFP-TAT synthesis circuit was combined with an in vivo inducible lysis circuit PviuA-E in E. coli to form an integrated antigen delivery system, the resultant E. coli was proved to be able to lyse upon the induction of a mimic in vivo signal and thus release intracellular EGFP-TAT intensively, which were assumed to undergo a more efficient intracellular delivery by CPP to evoke protective immune responses. Based on the established antigen delivery system, the protective antigen gene flgD from an invasive intracellular fish pathogen Edwardsiella tarda EIB202, was applied to establish an E. coli recombinant vector vaccine (Ma et al., 2014).
• Immunization Route: Intramuscular injection (i.m.)

Zebrafish Response

• Vaccine Immune Response Type: VO_0000287
• Efficacy: BL21(DE3)/pUTa-E + pET28a-FT conferred an significantly better immune protection (RPS of 63%) than the other control strains. This result suggested fused cell-penetrating peptides (CPPs) could facilitate the internalization of antigens, released by the lysed bacteria, into the immune-related cells, such as macrophage, and in turn promote a more protective immune response in host (Ma et al., 2014).