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Vaccine Detail

KLH-s-FimH1-25 with CFA and then IFA
Vaccine Information
  • Vaccine Name: KLH-s-FimH1-25 with CFA and then IFA
  • Target Pathogen: Escherichia coli
  • Target Disease: Hemorrhagic colitis
  • Type: Subunit vaccine
  • Status: Research
  • Host Species for Licensed Use: Mouse
  • Antigen: key-hole limpet hemocyanin (KLH)-conjugated s-FimH1–25FimH peptide 1-25 aa (Thankavel et al., 1997)
  • FimH from E. coli str. K-12 substr. MG1655 gene engineering:
    • Type: peptide 1-25 residues synthesis
    • Description:
    • Detailed Gene Information: Click Here.
  • Immunization Route: intramuscularly and subcutaneously
Host Response

Mouse Response

  • Immune Response: Each mouse was injected intramuscularly and subcutaneously with 150 mg of KLH-conjugated synthetic peptide emulsified in CFA. 4 wk later, each animal was boosted with 150 mg of the same immunogen emulsified in incomplete Freund’s Adjuvant (Thankavel et al., 1997).
  • Challenge Protocol: 5 d after intravesicular challenge with E. coli CI5 (50 ml of 1 3 109 CFU/ml PBS bacterial suspension), the bladder from each mouse was homogenized and the CFU determined (Thankavel et al., 1997).
  • Efficacy: in vivo E. coli colonization in the bladders of mice actively immunized with synthetic FimH1–25 was significantly reduced (Thankavel et al., 1997).
References
Thankavel et al., 1997: Thankavel K, Madison B, Ikeda T, Malaviya R, Shah AH, Arumugam PM, Abraham SN. Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. The Journal of clinical investigation. 1997; 100(5); 1123-1136. [PubMed: 9276729].