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Vaccine Detail

ADS-MVA vaccine
Vaccine Information
  • Vaccine Name: ADS-MVA vaccine
  • Target Pathogen: SARS-CoV
  • Target Disease: Severe Acute Respiratory Syndrome (SARS)
  • Type: Recombinant vector vaccine
  • Status: Research
  • Host Species for Licensed Use: None
  • Host Species as Laboratory Animal Model: mouse
  • Antigen: S protein(Chen et al., 2005)
  • S protein gene of SARS-CoV gene engineering:
    • Type: Recombinant vector construction
    • Description:
    • Detailed Gene Information: Click Here.
  • Immunization Route: Intramuscular injection (i.m.)
  • Description: Recombinant live-attenuated modified vaccinia virus Ankara (MVA) had full-length SARS-CoV envelope Spike (S) glycoprotein gene was introduced into the deletion III region of the MVA genome.
    (Chen et al., 2005)
Host Response

Macaque Response

  • Vaccination Protocol: immunized intramuscular injection twice with a 4-week interval. 1 × 10^8 TCID50 for the first immunization and a dose of 3 × 10^8 TCID50 for the second injection. (Chen et al., 2005)
  • Immune Response: testing hosts generated high levels of neutralizing antibodies after 2 vaccinations
    (Chen et al., 2005)
  • Challenge Protocol: immunized on days 0 and 28 via intranasal injection before challenged after second immunization on day 28 with 10^5 TCID50 of pathogenic SATS-CoVPUMC01.
    (Chen et al., 2005)
  • Efficacy: likely protected (Chen et al., 2005)
  • Description: After virus challenge, SARS-CoV shedding detected by RT-PCR was only detected in the nasopharyngeal specimens of one of the four ADS-MVA immunized animals (Rh0413) on day 2 after virus challenge. No virus shedding was detectable on days 4 and 6 postchallenge in these four macaques. SARS-CoV could not be isolated from the lung specimens of ADS-MVA-immunized macaques on day 7 postchallenge. (Chen et al., 2005)

Mouse Response

  • Host Strain: Balb/c (Chen et al., 2005)
  • Host age: 6-8 weeks (Chen et al., 2005)
  • Host gender: Female (Chen et al., 2005)
  • Vaccination Protocol: immunized intramuscular injection twice with a 3-week interval. Two mice were given 2 × 106 TCID50 of the vaccine, and six mice received 2 × 10^7 TCID50.(Chen et al., 2005)
  • Immune Response: testing hosts generated high levels of neutralizing antibodies (Chen et al., 2005)

Rabbit Response

  • Vaccination Protocol: immunized intramuscular injection twice with a 3-week interval (Chen et al., 2005)
  • Immune Response: testing hosts generated high levels of neutralizing antibodies (Chen et al., 2005)

Ferret Response

  • Host age: 6-10 weeks (Weingartl et al., 2004)
  • Host gender: Male (Weingartl et al., 2004)
  • Vaccination Protocol: Each ferret was immunized with rMVA-S (ferrets 7 to 9), n day 0 with a dose of 1e8 PFU of the corresponding virus per ferret by intraperitoneal and subcutaneous routes, and a booster immunization was given on day 14 with the same regimen.(Weingartl et al., 2004)
  • Immune Response: Neutralizing activity was detected in sera along with a corresponding immunoglobin G titer collected from all three ferrets 7 days after booster immunization with rMVA-S virus, while the titer declined to undetectable level 14 days after the booster (Weingartl et al., 2004).
  • Side Effects: Ferrets immunized with rMVA-S (particularly ferret 9) developed severe periportal and panlobular mononuclear hepatitis in contrast to only mild periportal mononuclear hepatitis was observed in control ferrets (Weingartl et al., 2004) .
  • Challenge Protocol: Ferrets were challenged with 1e6 PFU of the SARS-CoV Tor2 isolate by the intranasal route(Weingartl et al., 2004).
  • Description: Study shows correlation with liver damage but does not definitely proof it is caused as SARS-CoV in ferrets also damage liver.(Weingartl et al., 2004)
References
Chen et al., 2005: Chen Z, Zhang L, Qin C, Ba L, Yi CE, Zhang F, Wei Q, He T, Yu W, Yu J, Gao H, Tu X, Gettie A, Farzan M, Yuen KY, Ho DD. Recombinant modified vaccinia virus Ankara expressing the spike glycoprotein of severe acute respiratory syndrome coronavirus induces protective neutralizing antibodies primarily targeting the receptor binding region. Journal of virology. 2005; 79(5); 2678-2688. [PubMed: 15708987].
Weingartl et al., 2004: Weingartl H, Czub M, Czub S, Neufeld J, Marszal P, Gren J, Smith G, Jones S, Proulx R, Deschambault Y, Grudeski E, Andonov A, He R, Li Y, Copps J, Grolla A, Dick D, Berry J, Ganske S, Manning L, Cao J. Immunization with modified vaccinia virus Ankara-based recombinant vaccine against severe acute respiratory syndrome is associated with enhanced hepatitis in ferrets. Journal of virology. 2004; 78(22); 12672-12676. [PubMed: 15507655].