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Vaccine Detail

S. pneumoniae ClpP protein Vaccine
Vaccine Information
  • Vaccine Name: S. pneumoniae ClpP protein Vaccine
  • Target Pathogen: Streptococcus pneumoniae
  • Target Disease: Pneumonia
  • Vaccine Ontology ID: VO_0004006
  • Type: Subunit vaccine
  • Status: Research
  • Antigen: ATP-dependent caseinolytic protease (ClpP)
  • ClpP gene engineering:
    • Type: Recombinant protein preparation
    • Description:
    • Detailed Gene Information: Click Here.
  • Adjuvant: aluminum hydroxide vaccine adjuvant
  • Immunization Route: Intraperitoneal injection (i.p.)
Host Response

Mouse Response

  • Host Strain: BALB/c
  • Vaccination Protocol: Female BALB/c mice, weighing 16 to 18 g, were immunized three times at 14-day intervals with 10 µg of protein in alum adjuvant (3:1 [vol/vol]) of either ClpP protein in alum adjuvant or control protein in alum. Mice were boosted intraperitoneally with the same doses on days 14 and 28 (Wu et al., 2010).
  • Challenge Protocol: For the intraperitoneal challenge models, at 2 weeks after the last immunization BALB/c mice were administrated the virulent pneumococcal strain D39 at 1,500 times the 50% lethal dose (LD50; 1.5 x 105 CFU) or 150 times the LD50 (2.0 x 104 CFU). In the focal pneumonia models, BALB/c mice were intranasally inoculated with pneumococcal strain 31614 (serotype 14, 1.5 x 106 CFU) or 31693 (serotype 19F, 107 CFU). For intranasal challenge with pneumococcal strain D39, mice inspired the virulent pneumococcal strain D39 at 104 (7.5 x 107 CFU) or 103 (6.0 x 106 CFU) times the LD50 in a volume of 40 µl under injection anesthesia (Wu et al., 2010).
  • Efficacy: Compared to the control protein, ClpP reduced the median bacterial load nearly 100-fold in the focal pneumonia model (Wu et al., 2010).
References
Wu et al., 2010: Wu K, Zhang X, Shi J, Li N, Li D, Luo M, Cao J, Yin N, Wang H, Xu W, He Y, Yin Y. Immunization with a combination of three pneumococcal proteins confers additive and broad protection against Streptococcus pneumoniae Infections in Mice. Infection and immunity. 2010; 78(3); 1276-1283. [PubMed: 20038538].